Coding

Part:BBa_K3221102:Design

Designed by: Liu Yuxi   Group: iGEM19_SCU-China   (2019-09-30)


CNS1-linker-CNS2 coding sequence.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 3490
    Illegal SpeI site found at 711
    Illegal SpeI site found at 1240
    Illegal SpeI site found at 1894
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 711
    Illegal SpeI site found at 1240
    Illegal SpeI site found at 1894
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1288
    Illegal BglII site found at 1875
    Illegal BglII site found at 2293
    Illegal BamHI site found at 2377
    Illegal XhoI site found at 3484
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 3490
    Illegal SpeI site found at 711
    Illegal SpeI site found at 1240
    Illegal SpeI site found at 1894
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 3490
    Illegal SpeI site found at 711
    Illegal SpeI site found at 1240
    Illegal SpeI site found at 1894
    Illegal AgeI site found at 1354
    Illegal AgeI site found at 3553
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We bulit this part by cutting off the terminator downstream of CNS1 and promoter upstream of CNS2 with different enzymes.

Source

The original sequence of CNS1 and CNS2 comes from NCBI. The samples used for experiments are artificially synthesized by Sangong Biotech.


References

Xia Y , Luo F , Shang Y , et al. Fungal Cordycepin Biosynthesis Is Coupled with the Production of the Safeguard Molecule Pentostatin[J]. Cell Chemical Biology, 2017:S2451945617303276.